Loading Control–WB不可或缺的武器
内参的使用――WB过程监测以及目的蛋白定量的标准！
在WB试验中中，最重要和最不可或缺的对照就是内参照（Loading Control，Internal Control）。内参照的使用有两个方面的作用：
- 是检测整个WB实验过程及体系是否正常工作，如果一个实验连内参照都出不料阳性结果的话（在内参抗体有效的情况下），那么这个体系就是存在问题的了
- 半定量的标准：内参一般选择的管家基因编码表达的蛋白，在很多组织和细胞中中都是稳定表达的，因此可以作为检测靶蛋白表达量的标准。
Rabbit polyclonal to beta actin (ab8227) at 1/5000.
Lane 1: HeLa whole cell extract (20ug);
Lane 2: yeast cell extract (20ug)
Lane 3: mouse brain tissue lysate (20ug).
Developed using Goat anti-rabbit IgG (HRP) (ab6721) at 1/2000.
Don’t be a cowboy, use a Loading Control!
WB常用内参及其技术参数：
| 内参名称 | 分子量大小 | 适用范围 |
| beta-actin | 43kDa | 胞浆和全细胞 |
| GAPDH | 30-40kDa | 胞浆和全细胞 |
| Tubulin | 55kDa | 胞浆和全细胞 |
| VCDA1/Porin | 31kDa | 线粒体 |
| COXIV | 16kDa | 线粒体 |
| TBP | 38kDa | 细胞核 |
Abcam作为全球最大抗体供应商，拥有非常齐全的Loading Control，绝对能满足您WB的需求！
| 目录号 | 产品描述 | 规格 | 价格 |
| ab6276 | beta Actin antibody [AC-15]-loading control | 100 ul | 3968 |
| ab8226 | beta Actin antibody [mAbcam 8226] - Loading Control | 100ug | 3968 |
| ab8226 | beta Actin antibody [mAbcam 8226] - Loading Control | 25ug | 1380 |
| ab8227 | beta Actin antibody - Loading Control | 50ul | 3968 |
| ab13822 | beta Actin antibody - Loading Control | 100ug | 3450 |
| ab9484 | GAPDH antibody [mAbcam 9484] - Loading Control | 200ug | 3968 |
| ab9485 | GAPDH antibody - Loading Control | 100ug | 3795 |
| ab9485 | GAPDH antibody - Loading Control | 25ug | 1553 |
| ab15859 | VDAC1 / Porin antibody - Mitochondrial Loading Control | 100ug | 3795 |
| ab15859 | VDAC1 / Porin antibody - Mitochondrial Loading Control | 25ug | 1380 |
| ab14734 | VDAC1 / Porin antibody [20B12] – Mitochondrial Loading Control | 100ug | 3450 |
| ab6046 | beta Tubulin antibody – Loading Control | 100ug | 3795 |
| ab6046 | beta Tubulin antibody – Loading Control | 25ug | 1380 |
| ab21057 | beta Tubulin antibody - Loading Control | 100ug | 3450 |
| ab4074 | alpha Tubulin antibody - Loading Control | 100ug | 3795 |
| ab4074 | alpha Tubulin antibody - Loading Control | 25ug | 1380 |
| ab7291 | alpha Tubulin antibody [DM1A] - Loading Control | 100ug | 3795 |
| ab7291 | alpha Tubulin antibody [DM1A] - Loading Control | 25ug | 1380 |
| ab14744 | COX IV antibody [20E8] - Loading Control | 100ug | 3795 |
| ab16056 | COX IV antibody - Mitochondrial Loading Control | 100ug | 3795 |
| ab16056 | COX IV antibody - Mitochondrial Loading Control | 25ug | 1380 |
| ab33985 | COX IV antibody - Mitochondrial Loading Control | 100ug | 3968 |
| ab818 | TBP antibody [1TBP18] - Nuclear Loading Control | 100ug | 3968 |
| ab62126 | TBP antibody [mAbcam 62126] - Nuclear Loading Control and ChIP Grade | 100ug | 3450 |
更多产品和技术资料，请登陆www.abcam.com或致电51AB查询
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